Journal: Journal of Food Biochemistry

Article Title: Liquiritin Ameliorates Palmitic Acid‐Induced Hepatic Steatosis in Mouse Primary Hepatocytes by Suppressing the VEGFA Signaling Pathway

doi: 10.1155/jfbc/5387785

Figure Lengend Snippet: Figure 1: LIQ reduces PA-induced lipid accumulation in MPHs. (a) Chemical structure of LIQ. (b) CCK-8 analysis of MPHs treated with diferent concentrations of LIQ for 24 h. (c) Intracellular TG contents of MPHs that were cotreated with 200 μM PA and the indicated doses of LIQ for 24 h. (d) ORO staining analysis of MPHs that were treated with varying concentrations of LIQ (0, 5, 25, and 50 μM) for 24 h in the absence and presence of 200 μM PA. Magnifcation 400x. (e) Nile red staining analysis of MPHs that were treated with varying con- centrations of LIQ. Scale bar 25 μm. Data were represented as mean ± standard error of three independent experiments. ∗∗∗p < 0.001 vs. control group. ##p < 0.01 vs. PA group.

Article Snippet: After washing three times with PBS for 5min each time, Nile redstained neutral lipids (red) and DAPI-stained nuclei (blue) were photographed with a Nikon fuorescence microscope (400x magnifcation, ECLIPSE, Ts2R-FL, Tokyo, Japan).

Techniques: CCK-8 Assay, Staining, Control